. 2018 Mar 28;27(12):2090–2100. doi: 10.1093/hmg/ddy113

Table 1.

Physiological parameters of isolated EDL muscles used in ex vivo force measurements

Parameter	C57BL/10	mdx	Dys^ΔH2-R15-mdx	Dys^{ΔH2-R19/ΔCT}-mdx	Dys^{ΔR4–23/ΔCT}-mdx	flµDys-mdx	Dys^{ΔR2–15/ΔR18–23/ΔCT}-mdx	Dp116-mdx	P-value
Body mass (g)	36.2±2.2	36.4±1.3	36.0±0.7	40.4±1.4	31.6±0.7^d	37.4±1.6	31.3±1.7^d	32.2±0.7^d	<0.001
EDL mass (mg)	13.1±0.3	18.3±0.8^a	12.3±0.4^g ^d	12.5±0.1^g ^d	11.9±0.3^g ^d	12.6±0.4^g ^d	10.7±0.3^a ^g ^d	20.0±0.7^a	<0.001
L_o (mm)	12.9±0.1	13.0±0.2	12.8±0.3	13.9±0.1	12.9±0.2	12.8±0.5	12.6±0.2	13.9±0.2	0.051
CSA (mm²)	2.2±0.1	3.2±0.2^a	2.1±0.1^g ^d	1.9±0.1^a ^d ^g	2.0±0.1^d ^e ^g	2.1±0.1^d ^e ^g	1.8±0.1^a ^d ^g	3.1±0.01^a	<0.001
P_o (mN)	480±8.9	408±13^a ^g	349±21^a ^g	378±24^a ^g	428±11^a ^b ^c ^d ^e ^g	363±7.3^a ^g	380±12^a ^g	354±29^a	<0.001
Specific P_o (N/cm²)	21.5±0.5	13.1±0.5^a	17.8±0.8^b ^g	20.3±0.9^b ^g	20.4±0.9^b ^g	19.2±0.8^b ^g	19.7±0.8^b ^g	11.6±1.1^a ^b	<0.001
ΔP₀ (%)	1.2±0.4	91.0±1.2^a	1.0±1.7	7.0±1.6	5.9±0.8	2.0±1.8	36.7±4.5^a ^b ^g	88.6±3.7^a	<0.001
Peak eccentric force (mN)	795±15	614±17^a	644±27^a	642±38^a	704±24^a	594±9.7^a	601±32^a	532±36^a	<0.001
Eccentric force loss (%)	4.6±0.5	87.0±1.1^a	6.2±4.4	11.8±1.5	13.7±1.5	8.3±2.8	35.4±4.2^a ^b ^g	80.0±4.3^a	<0.001
Active stiffness (N/m)	935±36	671±17^a	675±26^a	784±46^a	781±35^a	694±19^a	674±28^a	613±30^a	<0.001
Peak twitch (mN)	144±6.5	104±4.7^a	89.9±6.1^a	117±4.5^a ^c ^e	101±7.0^a ^c	92.2±5.5^a	116±3.7^a ^c ^e	110±6.3^a	<0.001
Twitch force development time (ms)	18.0±0.3	18.7±0.5	19.1±0.2^g	19.5±0.2^g	19.5±0.9^g	18.3±0.3	19.5±0.2^g	17.1±0.5	<0.001
Twitch ½ relaxation time (ms)	16.0±0.5	20.4±1.1^a ^g	22.4±0.5^a ^g	22.3±1.3^a ^g	19.5±1.6^g	20.7±0.9^a ^g	19.5±0.5^g	15.2±0.6	<0.001
Tetanic maximal rate of contraction (mN/s)	15.7±0.6	12.2±0.6^a	10.8±0.4^a	13.4±0.1^c	12.1±0.4^a	12.1±0.4^a	12.0±0.4^a	12.3±0.7^a	<0.001
Tetanic maximal rate of relaxation (mN/s)	23.2±0.6	19.1±1.1^a	16.7±1.3^a	16.8±0.9^a	20.8±0.6^f	18.0±0.6^a	16.5±0.9^a	16.7±1.5^a	<0.001

Values are mean±SEM.

L_o=optimal muscle length, CSA=physiological cross sectional area, P_o=maximal isometric tetanic force. ΔP_o=change in P_o after eccentric contractions. n≥4 for each line. Statistics were performed using one-way ANOVA with Bonferroni post-hoc t-test analyses with P<0.05 considered significant.

Significantly different from C57BL/10.

Significantly different from mdx.

Significantly different from Dys^ΔH2-R15-mdx.

Significantly different from Dys^{ΔH2-R19/ΔCT}-mdx.

Significantly different from flµDys-mdx.

Significantly different from Dys^{ΔR2–15/ΔR18–23/ΔCT}-mdx.

Significantly different from Dp116-mdx.