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(A, B) Cholesterol accumulation and (A, C) lysosomes in control and NPC patient fibroblasts. Cholesterol content was assessed using the naturally fluorescent antibiotic, filipin [3]. Lysosomal morphology (size, distribution) was assessed using lysotracker-red. 20× magnification. Scale bar = 50 μm. Error bars: Mean ± SD, n = 3 wells/condition.*P < 0.05 versus Control I, **P < 0.01 versus Control I, $P < 0.05 versus Control II, one-way ANOVA followed by Tukey’s test.
