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. 2018 May 28;9:1161. doi: 10.3389/fimmu.2018.01161

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Copyright © 2018 Campillo-Navarro, Leyva-Paredes, Donis-Maturano, Rodríguez-López, Soria-Castro, García-Pérez, Puebla-Osorio, Ullrich, Luna-Herrera, Flores-Romo, Sumano-López, Pérez-Tapia, Estrada-Parra, Estrada-García and Chacón-Salinas.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Mast cells release DNA in response to heat-killed but not live Mycobacterium tuberculosis (Mtb). (A) Representative micrographs of HMC-1 cells or bone marrow-derived mast cells (BMMC) unstimulated (control) or stimulated during 2 h with PMA, live Mtb, or heat-killed Mtb (HK-Mtb) at a MOI of 10. DNA was visualized after staining with SYTOX-Green. Scale bar 20 µm (magnification 400×). (B) Released DNA was quantified in unstimulated HMC-1 cells (control) or stimulated at indicated times with PMA, live Mtb, or HK-Mtb at a MOI of 10. Released DNA was partially digested with DNase I and quantified in supernatants with SYTOX Green I in a fluorometer. The graph represents the change in fluorescence ± SD of stimulated cells compared to control. ***p < 0.001 as indicated.