Figure 6.

Catalase deficient Mycobacterium tuberculosis (Mtb KatG-) induce release of MCETs. (A) HMC-1 cells were left unstimulated (Control) or stimulated with PMA, live Mtb, heat-killed Mtb (HK-Mtb), or with a Mtb katG-deleted strain (Mtb KatG−) for 90 min and H₂O₂ was evaluated. The graph represents the change in fluorescence ± SD of stimulated cells compared to unstimulated cells ***p < 0.001 as indicated. (B) Extracellular DNA was evaluated in HMC-1 mast cells after 2 h of stimulation with PMA, live Mtb, HK-Mtb, or Mtb katG−. The graph represents the change in fluorescence ± SD of stimulated cells compared to the control. ***p < 0.001, **p < 0.01 as indicated. (C) Representative micrographs of HMC-1 cells unstimulated (Control) or stimulated during 2 h with PMA, Mtb, HK-Mtb or Mtb KatG− at a MOI of 10. DNA was visualized after staining with SYTOX-Green. Scale bar 20 µm (magnification 400×).