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. 2018 May 29;13:3163–3176. doi: 10.2147/IJN.S156880

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© 2018 Lo et al. This work is published and licensed by Dove Medical Press Limited

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Gelatin zymography and Western blotting of MMP-2 expression and activity.

Notes: VSMCs were treated with Veh or pAuNPs (2 and 5 ppm) in the presence or absence of PDGF (10 ng/mL) at 37°C for 16 hours. The media were removed, centrifuged, and then analyzed by gelatin zymography and WB. The quantitation of MMP-2 activity based on similar zymographic results is shown in the lower panel (n=3).

Abbreviations: pAuNPs, physically synthesized gold nanoparticles; VSMC, vascular smooth muscle cell; PDGF, platelet-derived growth factor; MMP-2, matrix metalloproteinase-2; Veh, vehicle; WB, Western blotting.