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. 2018 May 29;13:3163–3176. doi: 10.2147/IJN.S156880

Figure 6.

Figure 6

Effect of AuNPs on VSMC adhesion to collagen.

Notes: Cells were treated with vehicle or the indicated AuNPs for 30 minutes and allowed to adhere to collagen-precoated dishes in the presence or absence of PDGF (10 ng/mL) for 1 hour. VSMC adhesion was analyzed by (AC) microscopy, MTT reduction, and (D) immunofluorescence microscopy and fluorometry (n=2–3). *P<0.05, **P<0.01, and ***P<0.001 compared with vehicle control. Scale bar =100 μm.

Abbreviations: 3-EG AuNPs, 1-mercapto-(triethylene glycol)methyl ether functionalized AuNPs; 4-EG AuNPs, (1-mercaptoundec-11-yl)tetra(ethyleneglycol) functionalized AuNPs; AuNPs, gold nanoparticles; pAuNP, physically gold nanoparticle; std AuNPs, standard unconjugated AuNPs; VSMC, vascular smooth muscle cell.