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. 2018 May 29;13:3163–3176. doi: 10.2147/IJN.S156880

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© 2018 Lo et al. This work is published and licensed by Dove Medical Press Limited

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Effect of pAuNPs on collagen-induced protein tyrosine and FAK phosphorylation and actin cytoskeleton reorganization in VSMCs.

Notes: (A) Cells treated with vehicle or pAuNPs were allowed to adhere to collagen-precoated dishes for the indicated durations. Then, the cells were analyzed by Western blotting, and protein phosphorylation was quantified by densitometry (n=3–4). *P<0.05 compared with control. (B) Suspended VSMCs pretreated with vehicle (control) or pAuNPs (5 ppm) for 0.5 hours were allowed to adhere to collagen-precoated glass coverslips for an additional 1 hour. The cells were analyzed by immunofluorescence microscopy using anti-phosphotyrosine, p-FAK Ab, phalloidin-FITC, and DAPI as described in the Materials and methods section. Arrows indicate that stress fibers were formed in the control cells but disappeared in some pAuNP-treated cells. Scale bar =100 μm.

Abbreviations: pAuNPs, physically synthesized gold nanoparticles; VSMC, vascular smooth muscle cell; FAK, focal adhesion kinase; Ab, antibody; FITC, fluorescein isothiocyanate; DAPI, 4′,6-diamidino-2-phenylindole; Veh, vehicle.