Figure 4.

MHC (1000 ng) (A) and Renilla (100 ng) (B) plasmids were transfected into HEK293T via SLNs (references 12–16).

Notes: Data are from three independent experiments performed in triplicate (mean ± SEM). ***P < 0.001. MHC, plasmid containing a minimal fos promoter and three copies of the MHC class I κB element. Composition of the engineered nanoparticles – reference ¹²: 400 mg stearic acid, 100 mg cholesteryl oleate, 600 mg octadecylamine, 100 mg poloxamer 188; reference ¹³: 300 mg stearic acid, 200 mg cholesteryl oleate, 600 mg octadecylamine, 100 mg poloxamer 188; reference ¹⁴: 200 mg stearic acid, 300 mg cholesteryl oleate, 600 mg octadecylamine, 100 mg poloxamer 188; reference ¹⁵: 100 mg stearic acid, 400 mg cholesteryl oleate, 600 mg octadecylamine, 100 mg poloxamer 188; reference ¹⁶: 0 mg stearic acid, 500 mg cholesteryl oleate, 600 mg octadecylamine, 100 mg poloxamer 188.

Abbreviations: MHC, major histocompatibility complex; SEM, standard error of the mean; SLNs, solid lipid nanoparticles.