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. Author manuscript; available in PMC: 2019 Mar 1.
Published in final edited form as: Gene Rep. 2017 Nov 24;10:123–134. doi: 10.1016/j.genrep.2017.11.009

Table 9.

Use of reference genes in RT-qPCR studies of human retinal endothelial cells in 72 articles published between 2009 and 2015 on the National Library of Medicine of National Institutes of Health PubMed database (www.ncbi.nlm.nih.gov/pubmed; search date = 28 February, 2017; search text = human retinal endothelial cell; languages filter = English). Some articles reported works that involved multiple treatments. Articles are listed in Supplementary Table 3.

Treatment Number of articles Reference genes
Identity Number Stable
> 1 1 NR Yes NR
Glucose/AGE 25 18S rRNA, ACTB, GAPDH, HPRT1, U6 snRNA 0 24 1 0 25
Hypoxia & hypoxic mimics 6 ACTB, GAPDH 0 5 1 0 6
VEGF 8 ACTB, GAPDH 0 8 0 0 8
Cytokines 12 18S rRNA, ACTB, GAPDH, TBP 0 12 0 0 12
Other proteins 8 18S rRNA, ACTB, GAPDH, HPRT1 0 6 0 0 6
Microbial mimics 3 GAPDH, HPRT1, PGK1, TBP 1 2 0 1 2
Enzymes 2 18S rRNA, HPRT1 0 2 0 0 2
Lipids 2 18S rRNA, GAPDH, PPIA 0 2 0 0 2
Transfection of nucleic acid 8 18S rRNA, ACTB, GAPDH 0 8 0 0 8
Drugs & chemicals 9 ACTB, GAPDH, PPIA 0 9 0 0 9
Shear stress 2 GAPDH 0 2 0 0 2
No treatment 3 ACTA, GAPDH, HPRT1 0 3 0 0 3

Abbreviations: AGE = advanced glycation end-products, VEGF = vascular endothelial growth factor, NR = not recorded. Full names of reference genes with abbreviations appear in Supplementary Table 2.