FIG. 2.

Sperm cells were held in RMMB CPM for at least 5 min without significant impact of solution toxicity on cell motility. Average post-thaw motility of sperm incubated with RMMB CPM at room temperature for various amounts of time before freezing is shown. The 60-min control was incubated for 60 min (in E400 without RMMB) at 25°C before adding RMMB and freezing with a 0.5-min equilibration time. Motility was assessd post-thawing, and an ordinary post hoc one-way ANOVA was performed using Tukey's multiple comparison test to the mean of each column with every other column. n = 27 for each of the test and control groups. No significant difference was found between control and 0.5–10 min test groups (labeled a). The average motility rate for the time points 15–60 min (labeled b, c, d, and e) was statistically significantly different to any of the average motilities labeled (a). The adjusted p-values for the control group versus 0.5, 1, 2, and 5 min test groups were 0.49<p < 0.9996, whereas p = 0.001 for control versus 10 min, and p < 0.0001 for control versus 15, 30, 45, and 60 min test groups. CPM, cryoprotective medium.