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. 2018 Jun 1;27(11):723–735. doi: 10.1089/scd.2018.0002

FIG. 3.

FIG. 3.

Protein detection of MAEL and TEX11 in fetal and adult ovarian tissue. (A) Quantitative mass spectrometry detection of MAEL and TEX11 at four stages of fetal development (developmental days 47, 108, 122, and 137) and in a representative adult tissue sample. Values presented are iBAQ values normalized as fold change with respect to the 137-day sample. (B) Immunofluorescence analysis of fixed and sectioned ovarian tissue reveals localization of MAEL (green) and TEX11 (yellow) to germ cell nests. MAEL and TEX11 were only detected in the oocytes of primordial and primary follicles in adult tissue, with representative follicles shown. Cells were counterstained with Hoechst (blue) to visualize DNA. Inset shows representative secondary only control image (merge). Scale bars, 100 μm. iBAQ, intensity-based absolute quantification.