Skip to main content
. 2018 Jun 1;27(11):723–735. doi: 10.1089/scd.2018.0002

FIG. 4.

FIG. 4.

Analysis of MAEL and TEX11 expression in human oogonial stem cells and IVD oocytes. (A) Gene expression analysis by RT-PCR of MAEL, TEX11, and ZP3 in two hOSC lines derived from two patients. Cells released into suspension contained IVD oocytes and were analyzed separately from cultured cells. DNA was run on a 1.5% agarose gel and stained with ethidium bromide. (B) Immunofluorescence staining of cultured hOSCs for MAEL, TEX11, and DDX4 revealed cytoplasmic staining for all three markers in adherent hOSCs. Insets show representative secondary only control images. Scale bar, 50 μm. (C) Spontaneously generated IVD oocytes also stained positively for MAEL, TEX11, and DDX4. Insets show representative secondary only control images. Scale bar, 20 μm. hOSC, human oogonial stem cell; IVD, in vitro derived; RT-PCR, real-time PCR.