FIG. 2.

Comparison of adriamycin and puromycin as agents for podocyte injury. (A) Mean (±SEM) dextran clearance is significantly increased by injection of 25 mg mL⁻¹, but not by 5 mg mL⁻¹, puromycin at 3 dpf (n = 8). (B) Neither 1 nor 2 mg mL⁻¹ adriamycin injected at 3 dpf has a significant effect on mean (±SEM) dextran clearance (n = 8). (C) First derivative of (A) showing the rate change in dextran clearance is greatest within the first 24 hpi with the first derivative of 25 mg mL⁻¹ puromycin being significantly greater than both controls and 5 mg mL⁻¹-treated fish. (D) 25 mg mL⁻¹ puromycin injected at 4 dpf also significantly decreases mean (±SEM) dextran clearance 24 hpi. (E) 2 mg mL⁻¹ adriamycin significantly reduces mean (±SEM) heart rate, and puromycin has no effect (n = 8). (F) Mean (±SEM) venous blood flow is decreased by both 2 mg mL⁻¹ adriamycin and 25 mg mL⁻¹ puromycin (n = 8). Two-way ANOVA, p-value summary: *<0.05; **≤0.002; ***≤0.0002.