FIG. 4.

H₂O₂ inhibits neural differentiation. H₂O₂ was added into differentiating C17.2 cells for 5 or 7 days. Protein levels of Tuj1 (A) and GFAP (B) determined by immunoblotting. The quantification of the data was shown in the bar graph below. (C) Immunostaining of Tuj1 in the NG and NG plus H₂O₂ groups. Bars = 100 μm. (D) Quantification of Tuj1 positive cell number. (E) Quantification of the neurite length of neurons. Flow cytometry analysis on Tuj1⁺ and GFAP⁺ cells (F, G). Experiments were repeated three times (n = 3). Values are the mean ± SE from three separate experiments. *Indicates significant differences (P < 0.05) compared to the normal glucose (5 mM) groups. Color images available online at www.liebertpub.com/scd