FIG. 7.

Blockage of ER stress by 4-PBA restores neural differentiation suppressed by hydrogen peroxide. Protein levels of BiP, CHOP (A) and Tuj1 (B) in the control group (normal glucose, 5 mM), 4-PBA group, H₂O₂ group (H₂O₂, 10 μM), and 4-PBA plus H₂O₂ groups. The quantification of the data was shown in the bar graph. Immunostaining of Tuj1 (C) or GFAP (D) in C17.2 cell treated with 4-PBA or H₂O₂ and quantification for numbers of Tuj1 or GFAP positive cells. Bars = 40 μm. All experiments were repeated three times (n = 3). Values are the mean ± SE from three separate experiments. *Indicates significant differences (P < 0.05) compared to the control (5 mM) group. Color images available online at www.liebertpub.com/scd