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. Author manuscript; available in PMC: 2018 Nov 23.
Published in final edited form as: Nature. 2018 May 23;557(7707):714–718. doi: 10.1038/s41586-018-0144-9

Figure 2. Collagen V delays proliferation and differentiation of satellite cells.

Figure 2

(a) EdU pulse (2h) of isolated satellite cells cultured for 32h: COLI (35%), COLVI (34%), COLV (18%); (n=4 mice, ≥250 cells, 2 wells/condition).

(b) Immunostaining of isolated satellite cells cultured for 72h. PAX7: 58%, 55% and 81%; Myogenin: 56%, 57% and 24% for COLI, COLVI and COLV, respectively (n=4 mice, ≥250 cells, 2 wells/condition).

(c) Experimental scheme for satellite cells plated overnight (o/n) before collagen treatment.

(d) Immunostainings of satellite cells incubated with collagens for 60h (n=3 mice, ≥200 cells, 2 wells/condition).

Error bars, mean ± SD; two-sided paired t-test; #p-value: two-sided unpaired t-test. Scale bar: 50μm.