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. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: Cancer Res. 2018 May 7;78(11):3054–3066. doi: 10.1158/0008-5472.CAN-17-3932

Figure 2. Mitotic stress underlies WEE1i cytotoxicity.

Figure 2

(A-B) S/G2 duration of MIA PaCa-2 FastFUCCI cells treated as indicated. At least 100 cells per condition were analysed. Data are represented as mean ± SEM. A one-way ANOVA analysis was performed, ****p≤0.0001.

(C) Cell cycle duration of MIA PaCa-2 FastFUCCI cells treated with DMSO or 10 nM GEM+300 nM MK1775. A total of 243 cells were analysed. Data are represented as mean ± SEM. A two-tailed t-test was performed, *p≤0.05, **p≤0.01, ****p≤0.0001.

(D) Percentage of MIA PaCa-2 FastFUCCI cells in the first and second cycles that underwent division or not following 10 nM GEM+300 nM MK1775. Fraction of non-dividing cells was further categorised according to cell fate. A total of 122 cells were analysed.