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. Author manuscript; available in PMC: 2018 Jun 4.
Published in final edited form as: J Cell Physiol. 2014 Mar;229(3):343–352. doi: 10.1002/jcp.24454

Fig. 4.

Fig. 4

Western blots showing tyrosine phosphorylation of Na,K-ATPase α1 catalytic subunit in cells treated with SNP (100 μM) (Part A). A typical time course is presented alongside a bar graph that shows band density results pooled from three independent experiments (mean ± SEM). Part B shows the influence of PP2 on SNP-induced tyrosine phosphorylation of the Na,K-ATPase α1 subunit. Western blot analysis was performed on samples obtained by homogenizing cells that had been pre-incubated with PP2 (10 μM) for 40 min before being exposed to SNP (100 μM) for 10 min in the continued presence of PP2. A typical Western blot of pY-10-Na,K-ATPase-α1 bands (upper) and total Na,K-ATPase α1 subunit bands (lower) detected simultaneously in the same samples alongside a bar graph (lower) that shows pY-10-Na,K-ATPase-α1 band density normalized to total Na,K-ATPase α1 (mean ± SEM) of three independent samples, are presented. *P < 0.05, **P < 0.01, and ***P < 0.001 indicate significant differences from control.