Table 2.
Primer name, primer sequence, polymorphic information content (PIC), amplified alleles, and linkage group of 49 primers
| Primer name | 5′ to 3′ Left primer sequence | 5′ to 3′ Right primer sequence | PIC | No. of alleles | Linkage group |
|---|---|---|---|---|---|
| SSR primers | |||||
| BM139 | TTAGCAATACCGCCA TGAGAG |
ACTGTAGCTCAA ACAGGGCAC |
0.68 | 5 | B02 |
| BM154 | TCTTGCGAC CGAGCTTCTCC |
CTGAATCTGAGGAA CGATGACCAG |
0.77 | 5 | B09 |
| BM157 | ACTTAACAAGGAATAG CCACACA |
GTTAATTGTTTCCAATA TCAACCTG |
0.43 | 2 | B10 |
| BM160 | CGTGCTTGGCGA ATAGCTTTG |
CGCGGTTCTGATCG TGACTTC |
0.57 | 3 | B07 |
| BM164 | CCACCACAAGGAGAA GCAAC |
ACCATTCAGGCCG ATACTCC |
0.44 | 2 | B02 |
| BM170 | AGCCAGGTGCAAGACC TTAG |
AGATAGGGAGCTGGT GGTAGC |
0.50 | 2 | B06 |
| BM175 | CAACAGTTAAAGGTCG TCAAATT |
CCACTCTTAGCA TCAACTGGA |
0.66 | 3 | B05 |
| BM181 | ATGCTGCGAGTTAA TGATCG |
TGAGGAGCAAACAGA TGAGG |
0.78 | 5 | B03 |
| BM211 | ATACCCACATGC ACAAGTTTGG |
CCACCATGTGCTCATGA AGAT |
0.50 | 2 | B08 |
| BMd2 | AGCGACAGCAAG AGAACCTC |
CAACAAACGGTGAT TGACCA |
0.76 | 7 | B02 |
| BMd9 | TATGACACCACTGGC CATACA |
CACTGCGACATGAG AGAAAGA |
0.49 | 2 | B04 |
| BMd16 | ATGACACCACTGGCC ATACA |
GCACTGCGACATGAG AGAAA |
0.74 | 4 | B04 |
| BMd17 | GTTAGATCCCGCCCA ATAGTC |
AGATA GGAAGGGCGTGGTTT |
0.66 | 3 | B02 |
| BMd25 | GCAGATCGCCTACTC ACAA |
CGTTGACGAGAAGCA TCAAG |
0.47 | 2 | B08 |
| BMd33 | TACGCTGTGAT GCATGGTTT |
CCTGAAAGTGC AGAGTGGTG |
0.72 | 5 | B11 |
| BMd36 | CATAACATCGAAGCC TCACAGT |
ACGTGCGTACGAATAC TCAGTC |
0.49 | 2 | B03 |
| BMd42 | TCATAGAAGATTTGT GGAAGCA |
TGAGACACGTA CGAGGCTGTAT |
0.48 | 2 | B10 |
| BMd53 | TGCTGACCAAGGAAAT TCAG |
GGAGGAGGCTTAAGC ACAAA |
0.50 | 2 | B05 |
| GATS91 | GAGTGCGGAAGCGA GTAGAG |
TCCGTGTTCCTCTGTCT GTG |
0.49 | 2 | B02 |
| K03289 | ATGACACCACTGGCC ATACA |
GCACTGCGACATGAG AGAAA |
0.36 | 2 | B04 |
| X74919 | GTTGCCACCGGTG ATAATCT |
GTGAGGCAAGAAGCC TTCAA |
0.43 | 2 | B05 |
| X80051 | AGTTAAATTATA CGAGGTTAGCCTAAATC |
CATTCCCTTCACACA TTCACCG |
0.49 | 2 | B09 |
| X96999 | CAAATCGCAACACCTC ACAA |
GTCGGAGCCA TCATCTGTTT |
0.49 | 2 | B03 |
| Primers developed from tentative consensus (TC) sequences | |||||
| 755-size | TCCATGTCCCCTACAGA TTAAGGA |
TCCAAAGCCTACAAA TGGAACCTA |
0.40 | 2 | B4 |
| 762-allele-indel | GGATTTGTACAGT TGAAGTGACTTCTG |
CAAGCCCTTGAATA CTGAGTGTGA |
0.29 | 2 | B3 |
| 822-SNP | GAAGGGAAGTAGC TCAAAGAGAAAGGCG |
AGAAGCCATGAAG ATGAAGAACTAGACC AATAGAAG |
0.43 | 2 | B1 |
| 909-SSR | GAGTGACATCAAGGA ACAGATTCTAAATTACC |
CACCCACCCCCGAA TTAAGTTGC |
0.47 | 2 | B1 |
| 1148-size | GACTGGAAACATGTTG AGAGAATGG |
AATGTGCAACTACAAC AGAGTGCAA |
0.43 | 2 | B2 |
| 1181-SNP | TTGTGGAGTTGG CGAGGGCG |
AATTTTGGTTTTGG TTATTGTTGTTATTTT CCCAG |
0.47 | 2 | B8 |
| 1286-SNP | ATGATGCCTTAT GGAATGCCCCGA |
TCCCCGTAGTA CTAATGTGCAGATCTCA AGAAT |
0.40 | 2 | B9 |
| 1564-CAPS | TGTTTCATTTGATC CCAAGCCTAT |
TTCAACCATACAGAGA ACACAGAAGG |
0.42 | 2 | B4 |
| 1639-SNP | AATCTTTGTTGGGTGT TGTAAAGTTTGGATA AAGA |
CAAGAAGTGTG AGACGTTCTTCCGCTG |
0.45 | 2 | B6 |
| 2285-SNP | GATATACAGGGAAGC ATTAGCACTCAATCC TGAT |
ACATGATGAGTTAC CCAAGCCCAAAAGAA |
0.19 | 2 | B11 |
| 2298-SSR | ATATCTACCGATC ATAAGCCTTATTAAG |
AAATGGAGAAGGG TTACCTTC |
0.37 | 2 | B7 |
| 2422-allele-indel | GGACCATTGGTACAT ATTACAGTATATA |
GACTACACAAGAAT GGACTATTAAGG |
0.45 | 2 | B5 |
| 2551-size-indel | GAATCTCGTTTTCCTG CTCAG |
GATCTTGTAAGACT TTACTTTTACATTAGGAG |
0.49 | 2 | B6 |
| 2647-allele-indel | TATTACCTTTTCATC CTATTTTACACCATG |
ATATGAAGCAGAA CCAATGACTTGTG |
0.46 | 2 | B1 |
| 2654-SSR | CTATGCTCTCAA GTACCGCAAATTGG |
GGTTCCTCCATTTCTTT GTCCAACTC |
0.33 | 2 | B3 |
| 2685-size-indel | GTGCTTGGAATT CGAATTGGAGGTG |
CGAAACCATGCTATCA ACACACATCAAC |
0.19 | 2 | B4 |
| Primers developed from nucleotide-binding site–leucine-rich repeat (NBS-LRR) sequence | |||||
| 0436 | GGCACCATCTC AGAAAAACAA |
TGATGAATGAA CGAATGGGA |
0.60 | 3 | – |
| 0536 | TTTAGGAAATTCAGA TTGAGATATTG |
TGATGAATGAA CGAATGGGA |
0.67 | 3 | – |
| 2353 | GCATCCATTCCGAA CAGAGT |
GCTGCATGTTT AGATTTTTCCA |
0.98 | 9 | – |
| 2555 | CAGCTCTTCCAAAGTTGGTG | GAATTTGTTGA GCAGTGCGA |
0.95 | 6 | – |
| 2356 | GCATCCATTCCGAA CAGAGT |
AAAGCATAAGC ACATTGTTCCA |
0.89 | 6 | – |
| 2657 | TGGATACAC CCATTCTTTCTTG |
GCAGATTGATTTTTA TTCAACGG |
0.98 | 7 | – |
| 2758 | GGAGAACCTCCT GCAACTGA |
TCTTTAAG AGAATTCGCGGC |
0.99 | 6 | – |
| 2859 | GTGAAGGTGGTTTG GCATTT |
TGCAGGAGGTTCTC CAGATT |
0.85 | 7 | – |
| 2960 | ATCTGGAGAACCTCCT GCAA |
ATTCGCGGCCTG TTAAATC |
0.84 | 4 | – |
| 3061 | GCATCCATTCCGAAG AGAGT |
AAATGCCGAAGCCT TGAATA |
0.47 | 2 | – |