Skip to main content
. 2018 Jun 4;13(6):e0198637. doi: 10.1371/journal.pone.0198637

Fig 1. Schematic representation of the FTO genomic locus (chr16:53,703,963–54,121,941).

Fig 1

(A) SNPs rs1421085 (C/T) and rs8050136 (A/C) are located in the first intron of FTO. (B, C) CRISPR/Cas9 technology was employed to convert ESC line H9 (heterozygous for both SNPs) to homozygosity for both alleles at rs1421085 (C/C or T/T) or rs8050136 (C/C or A/A). Positions of gRNA, PAM sequence and ssODN are indicated by thick lines in blue, purple and black, respectively. SNPs are given in green. Predicted Cas9 cut sites are indicated by red arrow heads.