Fig 2. Overview of experimental setup.

H9 cells underwent ± DMSO treatment for 24 hours before collection for nucleofection. After 48 hours of recovery after nucleofection, transfected cells were isolated via CD4 bead-based magnetic labeling. They were then seeded at clonal density in 10 cm dishes to facilitate picking of single clones into 96 well plates. After expansion of each clone into two wells of a 96 well plate, genomic DNA was extracted from one well per clone and sequence analysis was performed. Correctly targeted clones were further expanded for freezing, immunocytochemistry, and karyotyping.