Fig. 6.

The relation between p62 and Nrf2 leading to authophagy. U937 cells were transfected with a specific Nrf2 siRNA for 48 h and then incubated with 27-OH (10 μM) for 6 h (A). Other cells were incubated with 27-OH (10 μM) in the presence of a specific antibody against Nrf2 for 6 h (B) or 48 h (C). Untreated cells were taken as controls. Each blot is representative of three experiments. Histograms represent mean values ± S.D. of three experiments and they are expressed as percentage of control value. **p < 0.01 and ***p < 0.001 vs. untreated control group; ##p < 0.01 vs. 27-OH.