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Predicted size and oligomeric state of cPAC and truncated variants
HPLC-SEC measurements were performed in duplicate (n = 2) and predicted molecular weight averaged as described under “Experimental procedures.”
| Construct | HPLC-SEC measurement | Predicted monomer molecular mass |
|---|---|---|
| cPAC (full-length) | 143 ± 8 kDa | 77 kDa |
| cPAC(ΔR3) | 68 ± 5 kDa | 60 kDa |
| cPAC(ΔR2) | 66 ± 4 kDa | 58 kDa |
| cPAC(ΔRG) | 45 ± 4 kDa | 41 kDa |
| cPAC(ΔRGP) | 30 ± 2 kDa | 27 kDa |
