Figure 5.

[³H]Tyr uptake into HeLa cells. A, Western blot analyses of His₆-tagged human TyrRS-overexpressing HeLa cells. The sizes in kilodaltons of molecular markers are indicated at the left. B, [³H]Tyr uptake into His₆-tagged human TyrRS-overexpressing HeLa cells. Data are expressed as mean ± S.D. of five independent experiments. C, SDS-PAGE of purified human TyrRS protein. The gel was stained with Coomassie Brilliant Blue. The sizes in kilodaltons of molecular markers are indicated at the left. D, analysis of the effect of TyrRS protein addition to the cell culture medium on [³H]Tyr uptake into HeLa cells. All data are expressed as mean ± S.D. of at least three independent experiments.