Figure 5.

A–C, inhibition of Heregulin 1-β1–dependent Akt phosphorylation was evaluated in the breast cancer T47D (A), MDA-MB-361 (B), and bladder cancer RT-112 (C) cell lines using a multiplexed Ser(P)⁴⁷³/total Akt sandwich immunoassay kit. D and E, the similarity of inhibition of HRG1-β1–dependent Akt phosphorylation by unconjugated MEDI4276 (Bs2–39SH) and combination of the parental antibodies. HER2 expression was evaluated in tumor xenografts by IHC test (data not shown), and the IHC intensity scoring is shown in parentheses for each cell line.