Figure 4.

The bypass efficiencies and mutation frequencies of the O²-alkyldT lesions in HEK293T cells and the isogenic cells where the TLS polymerases were individually depleted by CRISPR/Cas9. a–c, shown are the bypass efficiencies (a) and the frequencies for the T→A (b) and T→G (c) mutations observed for the O²-alkyldT lesions. The data represent the mean and S.D. of results from three independent replication experiments. **, 0.001 < p < 0.01; *, 0.01 < p < 0.05. The p values were calculated using two-tailed, unpaired t test, and the values referred to the comparisons between WT and TLS polymerase-deficient HEK293T cells.