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. 2018 Apr 17;293(22):8508–8520. doi: 10.1074/jbc.RA118.002106

Figure 4.

Figure 4.

KPP1 is required for segregation of the duplicated basal bodies and flagella. A, co-immunostaining of cells with the L8C4 antibody to label the flagellum (F) and the YL 1/2 antibody to label the flagellum-associated mBB. NF, new flagellum; OF, old flagellum. Scale bar, 5 μm. B, quantitation of the flagellum and the mature basal body in control cells (−Tet) and KPP1 RNAi cells (+Tet) that were induced for 72 h. 200 cells of each cell type (1N1K/1N1eK, 1N2K, 2N2K, and 2N1K) were counted, and three repeats were conducted. Error bars indicate S.D. C, co-immunostaining of cells with the YL 1/2 antibody to label mBB and the anti-TbSAS-6 antibody to label both the mBB (arrows) and pBB (arrowheads). Scale bar, 5 μm. D, measurement of inter-basal body (BB) distance in control and KPP1 RNAi cells. The 1N1eK cells are S-phase cells containing an elongated kDNA and duplicated basal bodies (2mBB–2pBB). The inter-BB distance was defined by the distance between the two mBB–pBB pairs. A total of 100 cells of each cell type were used for measurement with the ImageJ software. ns, no statistical significance; *, p < 0.05; ***, p < 0.001.