Figure 3.

Effect of HIRAN on HLTF-fork binding. A and B, EMSAs of HLTF (A) or ΔHIRAN (B) binding to 25 nm DNA forks containing fully paired or two unpaired nucleotides at the 3′-end of the nascent leading strand. Bands representing discrete protein-DNA complexes are labeled to the right of each gel. Quantitation of the percentage of each shifted band and the total from three experiments (mean ± S.D.) is shown to the right of a representative gel. The DNA remaining in the well (topmost band of each gel) was not included in the quantification. Asterisks represent the location of fluorescein tags.