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Attachment of InvD-coated latex beads to HEp-2 cells. A, latex beads (green) coated with InvA500 (positive control, corresponding to the extracellular part of InvA encompassing residues Pro-500–Ile-985 with four BIg domains and the adhesion domain), InvD1640 or BSA (negative control) were incubated with HEp-2 cells (HEp-2, ATCC® CCL-23TM). B, attached beads per cell were quantified after removal of unattached beads. Numbers in parentheses correspond to # beads/# of total cells.
