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. 2018 Mar 13;293(22):8672–8690. doi: 10.1074/jbc.RA117.001068

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Figure 7. — InvD interacts with Igs. A and B, homogenized extract of mouse intestines (A) and mouse Peyer's patches (B) mixed with 3×FLAG-tagged InvD1640 or ΔAD-InvD was subjected to FLAG pulldown assay. When using InvD1640 as bait, 25-kDa (in A and B) and 50-kDa (in B) bands were co-purified that were identified as the immunoglobulin light and heavy chain of antibodies. These bands were, however, not co-purified with the negative control ΔAD–InvD, indicating that the interaction is mediated by the adhesion domain of InvD. C, analysis of the interaction of InvD1640 with human IgG by microscale thermophoresis. InvA500 or ΔAD-InvD was used as negative control. Data shown are the average of three (InvD1640; ΔAD-InvD) or two (InvA500) technical repeats.