Table 2.
Plasmids used in this study
| Plasmid | Description | Ref. |
|---|---|---|
| pAM014 | pRS202 (URA3) containing 13xMYC-STP1 (1–114) (C44S C85S)-ZZ-6xHA | This work |
| pAM016 | pRS202 (URA3) containing 13xMYC-STP1 (1–114) (C44S C80S C85S)-ZZ-6xHA | This work |
| pAM017 | pRS202 (URA3) containing 13xMYC-STP1 (1–114) (C44S C80S)-ZZ-6xHA | This work |
| pAM018 | pRS202 (URA3) containing 13xMYC-STP1 (1–114) (C44S C80S C85S V82C)-ZZ-6xHA | This work |
| pAM020 | pRS202 (URA3) containing 13xMYC-STP1 (1–114) (C44S C80S C85S S86C)-ZZ-6xHA | This work |
| pAM021 | pRS202 (URA3) containing 13xMYC-STP1 (1–114) (C44S C80S C85S M87C)-ZZ-6xHA | This work |
| pAM022 | pRS202 (URA3) containing 13xMYC-STP1 (1–114) (C44S C80S C85S S90C)-ZZ-6xHA | This work |
| pAM029 | pMB31 containing 13xMYC-STP1 (V82G)-6xHA | This work |
| pAM030 | pMB31 containing 13xMYC-STP1 (V82D)-6xHA | This work |
| pAM046 | pMB31 containing 13xMYC-STP1 (V82S)-6xHA | This work |
| pAM047 | pMB31 containing 13xMYC-STP1 (V82K)-6xHA | This work |
| pAM048 | pMB31 containing 13xMYC-STP1 (V82A)-6xHA | This work |
| pAM049 | pMB31 containing 13xMYC-STP1 (V82F)-6xHA | This work |
| pAM050 | pMB31 containing 13xMYC-STP1 (C85A)-6xHA | This work |
| pAM051 | pMB31 containing 13xMYC-STP1 (C85S)-6xHA | This work |
| pAM052 | pMB31 containing 13xMYC-STP1 (C85L)-6xHA | This work |
| pAM053 | pMB31 containing 13xMYC-STP1 (C85F)-6xHA | This work |
| pAM054 | pMB31 containing 13xMYC-STP1 (C85D)-6xHA | This work |
| pAM055 | pMB31 containing 13xMYC-STP1 (C85K)-6xHA | This work |
| pAM056 | pMB31 containing 13xMYC-STP1 (S86A)-6xHA | This work |
| pAM057 | pMB31 containing 13xMYC-STP1 (S86F)-6xHA | This work |
| pAM058 | pMB31 containing 13xMYC-STP1 (S86K)-6xHA | This work |
| pAM059 | pMB30 containing 13xMYC-STP2 (V90G)-6xHA | This work |
| pAM060 | pMB30 containing 13xMYC-STP2 (V90D)-6xHA | This work |
| pAM061 | pMB30 containing 13xMYC-STP2 (C93A)-6xHA | This work |
| pAM062 | pMB30 containing 13xMYC-STP2 (C93D)-6xHA | This work |
| pAM063 | pMB30 containing 13xMYC-STP2 (V90S)-6xHA | This work |
| pAM064 | pMB30 containing 13xMYC-STP2 (V90K)-6xHA | This work |
| pAM067 | pMB30 containing 13xMYC-STP2 (V90L)-6xHA | This work |
| pAM068 | pMB30 containing 13xMYC-STP2 (A94F)-6xHA | This work |
| pAM069 | pMB30 containing 13xMYC-STP2 (C93F)-6xHA | This work |
| pAM080 | pRS202 (URA3) containing 13xMYC-STP2 (1–102) (C59S C60S)-ZZ-6xHA) | This work |
| pAM081 | pRS202 (URA3) containing 13xMYC-STP2 (1–102) (C59S C60S C93S A94C)-ZZ-6xHA | This work |
| pAM105 | pCA177 containing SSY5 (A381L)-6xHA-KlTRP1 | This work |
| pAM106 | pCA177 containing SSY5 (A381K)-6xHA-KlTRP1 | This work |
| pAM107 | pCA177 containing SSY5 (A381F)-6xHA-KlTRP1 | This work |
| pAM108 | pCA177 containing SSY5 (A381D)-6xHA-KlTRP1 | This work |
| pAM109 | pCA177 containing SSY5 (A381C)-6xHA-KlTRP1 | This work |
| pAM110 | pCA177 containing SSY5 (A381S)-6xHA-KlTRP1 | This work |
| pAM111 | pCA177 containing SSY5 (F634D)-6xHA-KlTRP1 | This work |
| pAM112 | pCA177 containing SSY5 (F634A)-6xHA-KlTRP1 | This work |
| pAM113 | pCA177 containing SSY5 (F634G)-6xHA-KlTRP1 | This work |
| pAM114 | pCA177 containing SSY5 (F634S)-6xHA-KlTRP1 | This work |
| pAM115 | pCA177 containing SSY5 (F634C)-6xHA-KlTRP1 | This work |
| pAM116 | pCA177 containing SSY5 (H661G)-6xHA-KlTRP1 | This work |
| pAM117 | pCA177 containing SSY5 (H661V)-6xHA-KlTRP1 | This work |
| pAM118 | pCA177 containing SSY5 (G671S)-6xHA-KlTRP1 | This work |
| pAM119 | pCA177 containing SSY5 (G671T)-6xHA-KlTRP1 | This work |
| pAM120 | pCA177 containing SSY5 (G671D)-6xHA-KlTRP1 | This work |
| pAM130 | pCA204 containing STP1 (C85L)-13xMYC-kanMX | This work |
| pCA147 | pEG202 (HIS3) KmR G418R containing PADH1-STP (1–124)-LexA | This work |
| pCA177 | pRS316 (URA3) containing SSY5-6xHA-KlTRP1 | This work |
| pCA204 | pRS317 (LYS2) containing STP1-13xMYC-kanMX | This work |
| pCA215 | pRS316 (URA3) containing SSY5 (H465A)-6xHA-KlTRP1 | This work |
| pCA216 | pRS316 (URA3) containing SSY5 (D545A)-6xHA-KlTRP1 | This work |
| pCA217 | pRS316 (URA3) containing SSY5 (S640A)-6xHA-KlTRP1 | This work |
| pCA218 | pRS316 (URA3) containing PTDH3-SSY5–6xHA-KlTRP1 | This work |
| pCA221 | pEG-KT (leu2-d) KlTRP1-loxP-GAL1-loxP-ZZ-TEV-STP1 (63-125)-GST | 16 |
| pCA222 | pEG-KT (leu2-d) KlTRP1-loxP-GAL1-loxP-ZZ-TEV-STP1 (70–125)-GST | This work |
| pCA237 | pEG-KT (leu2-d)KlTRP1-loxP-GAL1-loxP-ZZ-TEV-STP1 (80–125)-GST | This work |
| pCA246 | pEG-KT (leu2-d) KlTRP1-loxP-GAL1-loxP-ZZ-TEV-STP1 (70–99)-GST | This work |
| pCA254 | pEG-KT (leu2-d) KlTRP1-loxP-GAL1-loxP-ZZ-TEV-STP1 (76–90)-GST | This work |
| pCA260 | pRS202 (URA3) containing PTDH3-HAi-SSY5-GST-SBP-kanMX | 16 |
| pMB31 | pRS202 (URA3) containing 13xMYC-STP1–6xHA | 58 |
| pMB30 | pRS202 (URA3) containing 13xMYC-STP2–6xHA | 60 |
| pSH043 | pJG4-5 (TRP1) containing PGAL1-SSY5 (1–699) | 16 |
| pSH076 | pRS316 (URA3) containing SSY5 (1–381)-GST | 16 |
| pSH078 | pRS316 (URA3) containing SSY5 (382–699)-GST | This work |
| pSH091 | pRS316 (URA3) containing SSY5 (A382S)-GST | This work |
| pSH093 | pRS316 (URA3) containing SSY5 (A382C)-GST | This work |
| pSH095 | pEG202 (HIS3) KmR G418R containing PADH1--LexA-SSY5 (382–699) | This work |
| pSH096 | pJG4-5 (TRP1) containing B42-HA-SSY5 (1–381) | This work |
| pSH097 | pJG4-5 (TRP1) containing PGAL1-HAi-SSY5 (1–381) | This work |
| pSH098 | pRS202 (URA3) containing PTDH3-SSY5 (382–699)-GST | This work |
| pSH117 | pRS316 (URA3) containing SSY5 (K379A K380A S383A)-GST | This work |
| pSH120 | pRS316 (URA3) containing HAi-SSY5 (1–699)-GST | 50 |
| pSH125 | pJG4-5 (TRP1) containing B42-HA-SSY5 (382–699) | This work |
| pSH127 | pRS316 (URA3) containing SSY5 (K375A)-GST | This work |
| pSH128 | pRS316 (URA3) containing SSY5 (Δ379–387)-GST | This work |