Fig. 5.

Mutagenesis and enzymatic activity analysis of butyrate kinase (buk). A, The annotation of butyrate kinase in the butyrate biosynthesis pathway. B, The quantitative butyrylation sites in buk of C. acetobutylicum strain. C, Sequence alignment analysis the K239 of buk, from Exiguobacterium sibiricum (E. sibiricum), Bacillus cereus (B. cereus), Bacillus anthracis (B. anthracis), Bacillus subtilis (B. subtilis), Elusimicrobium minutum (E. minutum), Thermotoga maritima (T. maritima), Clostridium perfringens (C. perfringens) and C. acetobutylicum. D, Enzymatic activities of wild-type and K167Q, K239Q and K287Q mutant of buk. Purity of the wt and mutated proteins of buk shown by SDS-PAGE gel.