Fig. 1.

Phosphorylation motifs deduced from peptides with different modification levels following salt stress and verification of GmMYB173 as a substrate of GmCK2α. A, Phosphorylation motifs expressed as positional weight matrices extracted from the phosphopeptides in the Up group using motif-X software. B, Phosphorylation motifs extracted from the phosphopeptides in the Down group. C, GmMYB173 could be a substrate of GmCK2α. Phosphorylation assay was carried out as described in experimental procedure section. S59A and WT: recombinant proteins of the N-terminal 167 aa of GmMYB173-S59A and GmMYB173-WT fused to a Trx- and a 6 × His-tags at its N-terminal end, and a 6 × His-tag at its C-terminal end. Data indicated that the Ser59 is the unique site in GmMYB173.