HPLC-based analysis of cyanidin 3-arabinoside chloride (C3A) contents in transgenic roots of soybean.
A, The chromatographic spectrum observed from C3A standard separation. B, The chromatographic spectrum observed from HPLC separation of transgenic GmMYB173-OE roots without salt treatment. C–H The chromatographic spectrum observed from HPLC separation of transgenic GmCHS5-OE control (C), GmCHS5-OE salt stress (D), GmMYB173-OE control (E), GmMYB173-OE salt stress (F), GmMYB173S59D control (G), GmMYB173S59D salt stress (H) roots.