Fig. 2.

Effect of magnetic labeling on characteristics of conidia. Magnetic labeling of conidia has no effect on phagocytosis and acidification of phagolysosomes (PLs). A, The phagocytosis ratio was determined after 2 h coincubation of macrophages with magnetically labeled or unlabeled conidia of the wt and the pksP mutant. B, The ratio of acidified phagolysosomes was determined 2 h post-infection of macrophages with conidia. C, Integrity of the phagolysosomal membrane is not impaired by isolation of conidia-containing phagolysosomes. Cells were loaded overnight with fluorescent dextran beads. Conidia were labeled with CFW and added to the cells for 2 h. Cell lysis was performed by shearing the cell suspension and the lysate stained with DiD, specific for eukaryotic cell membranes. Images show a triple staining of particles with dextran, DiD and CFW indicating an intact phagolysosomal compartment.