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. 2018 Jun 4;7:e33286. doi: 10.7554/eLife.33286

Figure 4. Ascaroside production in deletion mutants of acyl-CoA oxidase genes acox-1.1 and acox-3.

Comparison of ascaroside and corresponding IC-ascaroside production in wild type, the acox-1.1(ok2257) deletion mutant, the acox-3(tm4033) deletion mutant, and the acox-1.1(ok2257);acox-3(tm4033) double deletion mutant. Data represent the mean ± SD of three independent experiments. Two-tailed, unpaired t-tests were used to determine statistical significance (*p≤0.05, **p≤0.01, ***p≤0.001).

Figure 4.

Figure 4—figure supplement 1. Ascaroside production in wild type, the acox-1.1(reb2[E434A]) catalytic mutant, the acox-1.4(reb6[E433A]) catalytic mutant, the acox-3(tm4033) deletion mutant, and double mutant and triple mutants.

Figure 4—figure supplement 1.

Log ratio of ascaroside production in wild type and mutants, relative to wild type. For ascarosides that could not be detected, their peak area was set at the detection limit of the LC-MS. Data represent the mean ± SD of three independent experiments.