Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (C. elegans) | acox-1.1 | WBGene00008564 | Other names: CELE_F08A8.1, acox-1 | |
| acox-1.2 | WBGene00008565 | Other names: CELE_F08A8.2, acox-2, drd-100 | ||
| acox-1.3 | WBGene00008566 | Other names: CELE_F08A8.3, acox-3 | ||
| acox-1.4 | WBGene00008567 | Other names: CELE_F08A8.4, acox-4 | ||
| acox-1.5 | WBGene00008167 | Other names: CELE_C48B4.1, acox-5, drd-51 | ||
| acox-1.6 | WBGene00010336 | Other names: CELE_F59F4.1, acox-6 | ||
| acox-3 | WBGene00019060 | Other names: CELE_F58F9.7, acox-6 | ||
| acs-7 | WBGene00007228 | Other name: CELE_C01G6.7 | ||
| maoc-1 | WBGene00017123 | Other name: CELE_E04F6.3 | ||
| dhs-28 | WBGene00000991 | Other name: CELE_M03A8.1 | ||
| daf-22 | WBGene00013284 | Other name: CELE_Y57A10C.6 | ||
| Gene (M. tuberculosis) | fadD6 | Gene ID: 887549 | ||
| Strains (C. elegans) | N2 | CGC | RRID:WB-STRAIN:N2_(ancestral) | wild type (Bristol) |
| RAB1 | doi: 10.1021/acschembio.7b01021 | backcrossed VC1785 acox-1.1(ok2257)I | ||
| RAB21 | doi: 10.1021/acschembio.7b01021 | backcrossed acox-1.4(tm6415)I | ||
| VC40449 | CGC | RRID:WB-STRAIN:VC40449 | acox-1.4(gk892586)I | |
| RAB22 | doi: 10.1021/acschembio.7b01021 | backcrossed acox-3(tm4033)IV | ||
| DR476 | CGC | RRID:WB-STRAIN:DR476 | daf-22(m130)II | |
| RAB24 | doi: 10.1021/acschembio.7b01021 | acox-1.1(reb2[E434A])I | ||
| RAB28 | doi: 10.1021/acschembio.7b01021 | acox-1.4(reb6[E433A])I | ||
| RAB30 | doi: 10.1021/acschembio.7b01021 | acox-1.1(ok2257)I; acox-3(tm4033)IV | ||
| RAB31 | doi: 10.1021/acschembio.7b01021 | acox-1.1(reb2[E434A])I; acox-1.4(reb6[E433A])I | ||
| RAB32 | doi: 10.1021/acschembio.7b01021 | acox-1.1(reb2[E434A])I; acox-3(tm4033)IV | ||
| RAB35 | this paper | acox-1.4(reb6[E433A])I; acox-3(tm4033)IV | ||
| RAB36 | this paper | acox-1.1(reb2[E434A])I; acox-1.4(reb6[E433A])I; acox-3(tm4033)IV | ||
| RAB37 | this paper | rebEx11 (Pacs-7::gfp::acs-7) | ||
| RAB38 | this paper | rebEx12 (Pacox-1.1::mcherry::acox-1.1; Pacs-7::gfp::acs-7) | ||
| RAB39 | this paper | rebEx13 (Pacox-1.1::mcherry::acox-1.1; Pacox-3::gfp::acox-3) | ||
| RAB40 | this paper | rebEx14 (Pacox-1.1::mcherry::acox-1.1; Pacs-7::gfp::acs-7) | ||
| Plasmids for protein expression | pET-22b-acs-7 | this paper | Cloning described in 'ACS-7 expression and mutagenesis' | |
| pET-22b-acs-7(E339A) | this paper | Cloning described in 'ACS-7 expression and mutagenesis' | ||
| pET-22b-acs-7(E339A,S186A,S187A) | this paper | Cloning described in 'ACS-7 expression and mutagenesis' | ||
| pET-16b-acox-1.1a | doi: 10.1073/pnas.1423951112 | |||
| pET-16b-acox-1.2 | doi: 10.1073/pnas.1423951112 | |||
| pET-16b-acox-3 | this paper | Cloning described in 'β-oxidation enzyme expression' | ||
| pACYCDuet-1-maoc-1 | this paper | Cloning described in 'β-oxidation enzyme expression' | ||
| pACYCDuet-1-dhs-28Δscp-2 | this paper | Cloning described in 'β-oxidation enzyme expression' | ||
| pET-16b-daf-22 | this paper | Cloning described in 'β-oxidation enzyme expression' | ||
| pET-28a-fadD6 | doi: 10.1021/acschembio.7b01021 | Cloning described in 'Synthesis of CoA-thioesters' | ||
| Plasmids for generating transgenic strains | pPD114.108-Pacox-1.1::mcherry::acox-1.1 | this paper | Cloning described in 'ACOX-1.1, ACOX-3, and ACS-7 localization and RNAi' | |
| pPD114.108-Pacs-7::gfp::acs-7 | this paper | Cloning described in 'ACOX-1.1, ACOX-3, and ACS-7 localization and RNAi' | ||
| pPD114.108-Pacox-3::gfp::acox-3 | this paper | Cloning described in 'ACOX-1.1, ACOX-3, and ACS-7 localization and RNAi' | ||
| Commercial kit | Q5 Site-Directed Mutagenesis Kit | New England Biolabs | E0554S | |
| Commercial enzymes | adenylate kinase | Sigma | M3003 | |
| pyruvate kinase and lactate dehydrogenase | Sigma | P0294 | ||
| Commercial compounds | LysoTracker Red (Deep Red) | Thermo Fisher | L12492 |