Figure 5.

Frondoside A enhances apoptotic cell death induced by 5-fluorouracil in HT-29 colon cancer cells. (A,B) Annexin V binding was carried out using Annexin V Dead Cell kit. HT-29 cells were treated with or without Frondoside A (0.5 μM) and indicated concentrations of 5-fluorouracil (0.5, 1, and 10 μM) individually and in combination for 48 h. Detached and adherent cells were collected and stained with Annexin V and 7-AAD, and then the events for early and late apoptotic cells were counted with the Muse Cell Analyzer as described in Materials and Methods. Data represent the mean ± S.E.M. of at least three independent experiments. Fr represents Frondoside A and 5-FU represents 5-fluorouracil.