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. 2018 May 19;10(5):643. doi: 10.3390/nu10050643

Figure 1.

Figure 1

Ln4 treatment inhibits lipid accumulation and enhances glucose uptake in 3T3-L1 adipocytes. (A,B) 3T3-L1 pre-adipocytes were seeded in 96-well plates and incubated with heat-killed cells (A) or freeze-dried broth (B) of 22 strains of lactic acid bacteria (LAB) (100 μg/mL, designated by a-v) during differentiation to adipocytes. Lipid droplets were detected by Oil Red O staining and measured by absorbance at 518 nm after dissolving in 2-propanol. *, p < 0.05; **, p < 0.01; ***, p < 0.001 vs. nM insulin (MDI) (ANOVA followed by Dunnett’s test) (C,D) Fully differentiated 3T3-L1 adipocytes were incubated with heat-killed cells (C), freeze-dried broth (D) of three strains of LAB (100 μg/mL) or insulin as a positive control (100 nM). Glucose uptake were detected by fluorescence-labeled glucose analog (2-NBDG) after 4 h incubation as described in Materials & Method section. Each bar represents mean ± SEM followed by 3 independent experiments. Asterisks (*, **, and ***) indicate statistical significance (one-way ANOVA/Dunnett’s test, p < 0.05, 0.01, and 0.001, respectively vs. MDI). (E) 3T3-L1 pre-adipocytes were seeded in 6-well plates and incubated with heat-killed cells (upper) and freeze-dried broth (bottom) of Ln4. The representative pictures of stained lipid droplets (×200) are presented. ND, non-differentiated group; MDI, differentiated group; O.D., Optical density; a, Lactobacillus brevis B151; b, Lactobacillus fermentum KCCM200060; c, Lactobacillus plantarum G72; d, Lactobacillus plantarum Ln4; e, Lactobacillus plantarum Lb41; f, Lactobacillus brevis G1; g, Lactobacillus brevis KCCM200080; h, Lactobacillus brevis KCCM200054; i, Leuconostoc citreum S.Pum19; j, Pediococcus pentosaceus SC28; k, Lactobacillus brevis KCCM200019; l, Leuconostoc Lactic KCCM202361; m, Leuconostoc lactis KCCM202369; n, Lactobacillus plantarum KCCM200656; o, Lactobacillus plantarum KCCM200693; p, Lactobacillus plantarum KCCM200650; q, Lactobacillus plantarum KCCM202303; r, Lactobacillus plantarum KCCM200655; s, Lactobacillus plantarum KCCM200661; t, Lactobacillus brevis KCCM202302; u, Lactobacillus brevis KCCM202399; v, Lactobacillus Buchneri KCCM200793.