Fig. 4.

Representative TEM images of C6-GSCs. A: Control cell showing the typical chromatin pattern of nucleus (N) with numerous microvilli (Mv) arising from the cell membrane and mitochondria (Mit). B, C and D: Early apoptosis in cells treated with Met for 24 h showing shrinkage, membrane blebs (Bl), vacuolar cytoplasm(V), and nuclear chromatin condensation(Cc). E and F: Late apoptosis in cells treated with 9-cis RA for 24 h showing abundant cytoplasmic vacuoles (V) and nuclear fragments (Nf) with few lipid droplets. G and H: Apoptotic bodies (Ab) and nuclear fragments (Nf) in C6-GSCs treated with Met+9-cis RA for 24 h.