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Characterization of exosomes. TEM of isolated vesicles: Electron micrographs display sets of distinct vesicles isolated from U87MG cells cultured under (A) normoxic conditions with average diameters of 71 ± 14 nm (C) while those derived from cells cultured under (B) hypoxic conditions had a slightly smaller average diameter of 61 ± 13 nm (C), (Scale bar: 100 nm). (D) Western blot analysis of equivalent amounts cell lysates and exosomes showed enrichment of CD63 in exosomes.
