Fig. 3.

Elevated levels of proteins detected in hypoxic exosomes. (A) Western blot analysis of exosomes isolated from serum free, conditioned media of U87MG cells cultured under hypoxic conditions. Among the proteins detected in hypoxic exosomes were TSP-1, LOX, ADAMTS1, and VEGF. (B-E) Quantification of the western blots showed a significant increase in levels of secreted TSP-1, LOX, ADAMTS1, and VEGF in hypoxic exosomes compared to normoxic exosomes. Hif-1α (F) and Galectin-1 (G) were found to be elevated in hypoxic cell lysates although the increase was not significant. β-actin was used as loading control. Error bars, SEM (*p < 0.05, **p < 0.01, ***p < 0.001, ns=non significant).