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. 2018 May 2;14:104–113. doi: 10.1016/j.bbrep.2018.03.008

Fig. S1.

Fig. S1

Confocal images of U87 cells. U87MG cells grown under normoxic (A) and hypoxic (B) conditions in presence of pimonidazole. FITC conjugated anti-pimonidazole antibody was used to detect pimonidazole-induced adducts in cells. Cells were counter-stained for Hif-1α. Hypoxic cells showed increased binding of pimonidazole and increased fluorescence for Hif-1α relative to cells cultured in normoxic conditions.