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Identified lysine-to-alanine mutations of recombinant αB-crystallin do not alter expression of formation of OCs. (A) The amino acid sequence of αB-crystallin with lysines that were substituted with alanine underlined. (B) SDS-PAGE showing soluble purified recombinant αB-crystallin substitution mutants. (C) Gel filtration analysis of wildtype and αB-crystallin substitution mutants showing the formation of similar OCs.
