Table 1.
Oligonucleotides sequences used for PCR and cloning.
| Name | Sequence (5′–3′)a |
|---|---|
| αB Forward | TTTCCATGGACATCGCCATC |
| αB Reverse | AACAAGCTTTCATTTCTTGGGGGCTGC |
| K72A For | CTGGAGGCGGACAGGTTCTCTGTC |
| K72A Rev | CCTGTCCGCCTCCAGGCGCATCTC |
| K82A For | GATGTGGCGCACTTCTCCCCAGAG |
| K82A Rev | GAAGTGCGCCACATCCAGGTTGAC |
| K90A For | GAACTCGCAGTTAAGGTGTTGGGAG |
| K90A Rev | CTTAACTGCGAGTTCCTCTGGGGAG |
| K92A For | AAAGTTGCGGTGTTGGGAGATGTG |
| K92A Rev | CAACACCGCAACTTTGAGTTCCTC |
| K103A For | CATGGAGCACATGAAGAGCGCCAG |
| K103A Rev | TTCATGTGCTCCATGCACCTCAATC |
| K121A For | CACAGGGCATACCGGATCCCAGCTG |
| K121A Rev | CCGGTATGCCCTGTGGAACTCCCTG |
| K150A For | CCAAGGGCACAGGTCTCTGGCCCTG |
| K150A Rev | GACCTGTGCCCTTGGTCCATTCAC |
| K166A For | AAGAGGCGCCTGCTGTCACCGCAG |
| K166 Rev | AGCAGGCGCCTCTTCACGGGTGATG |
| K174/175A Reverse | TATAAGCTTCTATGCCGCGGGGGCTGCGGTGAC |
a
Restriction endonuclease recognition sites are underlined.