Figure 6. The radiosensitizing effect of miR-513a-5p is increased by suppressing APE1.
Osteosarcoma cells with miR-513a-5p expression and control cells were first transfected with wildtype APE1 expression vector and irradiated with 4 Gy X-ray. The apoptosis of these cells was analyzed by flow cytometry after AnnexinV/PI staining. The representative flow cytometry is shown in (A) and the statistical results are shown in the bar graph in (B). An asterisk (*) indicates that the difference between the marked group and its vector transfected counterpart is statistically significant (p < 0.01). (C) The expression of APE1 and induction of γ-H2AX in cells with the indicated treatment after 4 Gy irradiation was determined by Western blot, and the representative blots and statistical results are shown in (D).