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. 2018 May 22;9(39):25529–25544. doi: 10.18632/oncotarget.25368

Figure 5. Silencing REDD1 reduces SEL-DEX inhibitory effect on mTOR-activity.

Figure 5

(A) MM.1S cells were transfected with either 40 nM REDD1 or control siRNA using Neon Transfection System as per manufacturer’s instruction. The siRNA transfected and non-transfected cells were treated for 24 hours with 200 nM selinexor and 100 nM DEX 48 hours post transfection. The cell lysates for evaluated by western blotting for the expression of mTOR pathway related genes. Densitometry analysis showed of statistically significant difference in the reduction of phospho (Ser-235/236) RPS6 and phospho (Ser-65) with the treatments between REDD1 silenced cells and cells transfected with control siRNA. (B) MM.1S cells were transfected with either 40 nM BCAT2 or control siRNA using Neon Transfection System and the cells were treated for 24 hours with 200 nM selinexor and 100 nM DEX for 48 hours post transfection. BCAT2 silencing didn’t significantly affect the impact of selinexor and DEX on mTOR targets.