Table 1. Sample characteristicsand CpG sequencing data of the four groups.
| n mean ± SD |
Control n=5 |
Obesity n=5 |
Family n=5 |
Cancer n=5 |
|---|---|---|---|---|
| Gender | ||||
| Female | 3 | 2 | 2 | 5 |
| Male | 2 | 3 | 3 | 0 |
| BMI | ||||
| ≤ 30 | 5 | 0 | 4 | 3 |
| >30 | 0 | 5 | 1 | 2 |
| Age | 35 ± 7 | 45 ± 5 | 31 ± 13 | 58 ± 8 |
| Raw reads | 34,560,581 ± 17,900,576 | 28,087,864 ± 3,628,196 | 46,728,944 ± 5,939,651 | 34,857,916 ± 5,649,474 |
| QC-passed reads | 24,300,490 ± 12,874,723 | 19,538,524 ± 2,733,127 | 16,917,641 ± 1,803,606 | 12,528,537 ± 1,986,199 |
| Mapping Efficiency | 69.9 ± 1.44 | 69.52 ± 2.31 | 72.56 ± 1.95 | 71.93 ± 0.88 |
| # of CpGs > 0 read | 6,328,477 ± 811,790 | 6,436,231 ± 697,978 | 7,458,949 ± 494,165 | 6,632,163 ± 726,277 |
| # of CpGs > 5 read | 4,184,837 ± 479,512 | 4,291,259 ± 242,916 | 4,037,734 ± 415,253 | 3,669,350 ± 342,216 |
| CpG Methylation % | 41.7 ± 0.63 | 41.51 ± 1.00 | 45.69 ± 2.07 | 42.6 ± 2.87 |
| CpG Coverage | 19.60 ± 10.06 | 15.27 ± 2.84 | 9.53 ± 1.14 | 9.60 ± 2.36 |
| # of CGI | 24,268 ± 323 | 24,294 ± 348 | 24,483 ± 143 | 24,186 ± 236 |
| # of CpGs on CGI | 2,186.183 ± 281,350 | 2,195,293 ± 254,964 | 2,324,162 ± 44,814 | 2,266,921 ±77,206 |
Note. BMI: body mass index; QC: quality control; CGI: CpG Island.