Fig. 4.

Expression of Wnt3a mRNA in developing mouse brain following maternal exposure to PGE₂. (A) The expression of Wnt3a was generally increased in most pups at E16 (L1-L3). (B) At E19, the expression of Wnt3a was increased in all three litters (L4-L6). (C) Two litters L7 and L8 had increased Wnt3a expression at P8, while L9 had decreased expression. Horizontal lines represent the mean value within each litter. (D) In the control mouse brain, Wnt3a expression is highest at E16 (p = 0.00092), and decreases throughout development in E19 (p = 0.0035) and P8 (p = 0.0050). Following PGE₂ exposure, mean Wnt3a expression from all pups is significantly greater at E16 (p = 0.0040), and remains elevated at E19 (p = 0.0022) and P8 (p = 0.040), from the normal expression in controls. Each value was averaged from animals from three litters and three separate experiments.