. 2018 May 14;7(2):235–247. doi: 10.1007/s40121-018-0202-5

Table 1.

Results of practical hand wash test with Escherichia coli according to EN1499

Test parameter		Reference soft soap			PVP-I			CHG
Product volume	Contact time (s)	Pre-value	Post-value	Log₁₀ reduction^a	Pre-value	Post value	Log₁₀ reduction^a	Pre-value	Post-value	Log₁₀ reduction^a
3 mL	15	6.05	3.24	2.80	5.99	1.90	4.09^b	6.03	0.81	5.22^b
	30	5.93	3.10	2.83	5.94	1.45	4.48^b	5.95	0.86	5.09^b
	60	5.82	2.96	2.86	5.91	1.12	4.97^b	6.00	0.86	5.14^b
5 mL	15	6.02	2.90	3.11	5.93	1.71	4.22^b	6.00	1.87	4.12^b
	30	5.89	3.14	2.75	5.81	1.49	4.32^b	5.97	1.64	4.33^b
	60	5.96	3.05	2.91	5.94	0.67	5.27^b	5.86	1.23	4.63^b

Each value represents the mean from 30 samples (left and right hands of 15 volunteers)

CHG chlorhexidine gluconate 4% hand cleanser, PVP-I povidone-iodine 7.5% hand cleanser

^aMean log₁₀ reduction of post-washing versus pre-washing samples

^bDemonstrated a significant difference versus the reference soft soap at the p = 0.01 level (one-sided), denoting that the test product passes the EN1499 test. The reference procedure with soft soap always used 5 mL soap for 60 s contact time per EN1499 standard